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Image Search Results
Journal: The EMBO Journal
Article Title: The eukaryotic replisome tolerates leading‐strand base damage by replicase switching
doi: 10.15252/embj.2020107037
Figure Lengend Snippet: Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea polyacrylamide gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
Article Snippet: Samples were heated to 95°C for 2 min and resolved on 15%
Techniques: Pulse Chase, Generated, Titration, Primer Extension Assay, Sequencing