polyacrylamide non denaturing gels Search Results


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Euromedex bis-acrylamide
Bis Acrylamide, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Roth GmbH polyacrylamide denaturing gels
Polyacrylamide Denaturing Gels, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alamo Gels Inc non-denaturing polyacrylamide slab gels
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FLOWGEN BIOSCIENCE LIMITED polyacrylamide gels
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IBI Scientific 10% non-denaturing polyacrylamide gel with 0.5× tbe running buffer
10% Non Denaturing Polyacrylamide Gel With 0.5× Tbe Running Buffer, supplied by IBI Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellvis Inc non-denaturing polyacrylamide gel
Non Denaturing Polyacrylamide Gel, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Grafix Inc non-denaturing polyacrylamide gel electrophoresis
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Fisher Scientific 15% polyacrylamide 7 m urea denaturing gels
Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea <t>polyacrylamide</t> gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
15% Polyacrylamide 7 M Urea Denaturing Gels, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hoefer non-denaturing polyacrylamide gel mini-gel plates thickness, 80
Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea <t>polyacrylamide</t> gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
Non Denaturing Polyacrylamide Gel Mini Gel Plates Thickness, 80, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Severn Biotech Limited 10% polyacrylamide/bis (37.5 : 1) gels with denaturing gradients from 30– 60
Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea <t>polyacrylamide</t> gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
10% Polyacrylamide/Bis (37.5 : 1) Gels With Denaturing Gradients From 30– 60, supplied by Severn Biotech Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Panomics Inc 6% polyacrylamide gel
Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea <t>polyacrylamide</t> gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
6% Polyacrylamide Gel, supplied by Panomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cambrex denaturing longranger polyacrylamide gels
Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea <t>polyacrylamide</t> gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.
Denaturing Longranger Polyacrylamide Gels, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea polyacrylamide gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.

Journal: The EMBO Journal

Article Title: The eukaryotic replisome tolerates leading‐strand base damage by replicase switching

doi: 10.15252/embj.2020107037

Figure Lengend Snippet: Outline of pulse chase reaction used in (B–E). Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E). Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea polyacrylamide gel. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken. Quantification of bypass 5 min after addition of the chase for the data in (E). Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.

Article Snippet: Samples were heated to 95°C for 2 min and resolved on 15% polyacrylamide (Bis‐Acrylamide 19:1—Fisher Scientific) 7 M urea denaturing gels.

Techniques: Pulse Chase, Generated, Titration, Primer Extension Assay, Sequencing